We did that a couple of years ago in Rick Firtel’s lab. We did a mCherry-H2B fusion. I believe it was the mouse gene. Rick retired and I moved on so that plasmid will need to be recreated but it worked well.



Ruedi



> On Mar 27, 2018, at 12:32, Sucgang, Richard S <[log in to unmask]> wrote:

> 

> An important consideration is that many of these dyes will be pumped out by the transporters, and the signal weakens as the cells sit. I seem to recall someone doing a GFP-histone fusion that can be used in this manner. 

> 

> 

>> On Mar 25, 2018, at 11:31 PM, 久保原禅 <[log in to unmask]> wrote:

>> 

>> Dear Dr.

>> You can stain the nucleus in living dicty cells with hoechst33342 (for example, final conc. 1 microg/ml for 30 min).

>> But the dye stains the cell walls of stalk cells (and probably spores), too. be careful.

>> 

>> Best wishes,

>> 

>> Yuzuru Kubohara, Ph.D.

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>> 

>> 2018-03-25 2:31 GMT+09:00 mona saad <[log in to unmask]>:

>> Dear all, 

>> could you recommend for me a fluorescent dye that color the nucleus in dicty in order to do imaging in living cells?

>> Thank you

>> 

>