Dear Dave,
This is ok with me. I also have red histone constructs that work quite
nicely. I can send them to the DSC.
Best wishes,
Annette
---
PD Dr. Annette Mueller-Taubenberger
BMC - LS Zellbiologie (Anatomie III)
Ludwig-Maximilians-Universität
Großhaderner Str. 9
82152 Martinsried-Planegg
Ph.: +49 89 218075-873 (office)
Ph.: +49 89 218075-861 (lab)
Am 2018-03-29 18:40, schrieb Knecht, David:
> I have both Annette’s histone-GFP construct and Doug Robinson’s
> NLS-td-Tomato constructs (which we use for nuclei) and can deposit
> them in Dictybase stock center assuming the labs agree. Dave
>
> Dr. David Knecht
> Professor , Department of Molecular and Cell Biology
> University of Connecticut
> 91 N. Eagleville Rd.
> U-3125
> Storrs, CT 06269-3125
> 860-486-2200
>
>> On Mar 29, 2018, at 11:06 AM, Chubb, Jonathan <[log in to unmask]>
>> wrote:
>>
>> We have an AX3-based cell line with H2B-mCherry knocked into the
>> rps30 gene on the duplication. It is even and bright.
>>
>> -------------------------
>>
>> FROM: DICTY <[log in to unmask]> on behalf of Meili,
>> Rudolf <[log in to unmask]>
>> SENT: 29 March 2018 15:34
>> TO: [log in to unmask]
>> SUBJECT: Re: [DICTY] Fluorescent dye for nucleus in living cells
>>
>> We did that a couple of years ago in Rick Firtel’s lab. We did a
>> mCherry-H2B fusion. I believe it was the mouse gene. Rick retired
>> and I moved on so that plasmid will need to be recreated but it
>> worked well.
>>
>> Ruedi
>>
>>> On Mar 27, 2018, at 12:32, Sucgang, Richard S <[log in to unmask]>
>> wrote:
>>>
>>> An important consideration is that many of these dyes will be
>> pumped out by the transporters, and the signal weakens as the cells
>> sit. I seem to recall someone doing a GFP-histone fusion that can be
>> used in this manner.
>>>
>>>
>>>> On Mar 25, 2018, at 11:31 PM, 久保原禅
>> <[log in to unmask]> wrote:
>>>>
>>>> Dear Dr.
>>>> You can stain the nucleus in living dicty cells with hoechst33342
>> (for example, final conc. 1 microg/ml for 30 min).
>>>> But the dye stains the cell walls of stalk cells (and probably
>> spores), too. be careful.
>>>>
>>>> Best wishes,
>>>>
>>>> Yuzuru Kubohara, Ph.D.
>>>>
>>
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>>>> Graduate School of Health and Sports Science
>>>> 1-1 Hiraga-Gakuendai, Inzai City, Chiba 270-1695
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>>>>
>>
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>>>>
>>>> 2018-03-25 2:31 GMT+09:00 mona saad <[log in to unmask]>:
>>>> Dear all,
>>>> could you recommend for me a fluorescent dye that color the
>> nucleus in dicty in order to do imaging in living cells?
>>>> Thank you
>>>>
>>>
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