Hi Stephen,
We purchase Formedium HL5 w/ glucose in 250 g containers (~15 at a time). It took about 2 months to get the 1st permit and our cost was $133. Annual renewals are $86. The permit is for a specific item(s) and not a blanket permit. You can get the form at: http://www.aphis.usda.gov/import_export/animals/downloads/vs16_3.pdf
Once we had the permit, it took about 1.5 mo to get the order from Formedium. Initially, not knowing about the permit process, our order sat in the Formedium office for a couple of months without action. Our first orders from Formedium did not require a permit and took about 2 months, so we did not realize there was a problem. I recommend tracking the order on a regular basis.
David
David Lindsey
Professor of Biology
Walla Walla University
College Place, WA 99324
509-527-2642
________________________________________
From: DICTY [[log in to unmask]] On Behalf Of Deen [[log in to unmask]]
Sent: Wednesday, January 06, 2010 8:00 AM
To: [log in to unmask]
Subject: Re: [DICTY - 242] Purchasing Media from Formedium/UK
Hi Stephen
Buying Formedium HL5 is cheaper than local producers, it is at least 10
times cheaper
you can get 6 kg for about $350-$450 (depending on the mix)
(see
http://www.formedium.com/usa/DictyosteliumDiscoideum/DictyosteliumDiscoideum.htm#HL5%20Medium%20including%20Glucose)
Getting permit is not a problem but it takes time, we submitted it in
Sep, 2009 and until now we have not got it (time taken by USDA is
workload dependent).
But procedure is easy all we have to do is submit a Vs-16 form to USDA
and i think we should pay about $138 fee (one time).
hope this information is useful.
regards
Deen
Paul Steimle P_STEIML wrote:
> Hi Stephen,
>
> A possible alternative is to use MP Biomedicals which offers an HL5 mix
> (see <http://www.mpbio.com/quick_search_results.php?q=hl5>), albeit only at
> the 454g scale and at about $444.
>
> However, they do not offer LoFlo medium.
>
> Hope this is helpful.
>
> -----------------------------------------------------------------------
> Paul Steimle
> Associate Professor of Biology
> University of North Carolina at Greensboro
> Greensboro, NC 27402
> Phone: 336-334-4949
>
>
>
> -----DICTY <[log in to unmask]> wrote: -----
>
>
> To: [log in to unmask]
> From: nimi vashi <[log in to unmask]>
> Sent by: DICTY <[log in to unmask]>
> Date: 01/05/2010 04:44PM
> Subject: Re: [DICTY - 240] Purchasing Media from Formedium/UK
>
> Dear Stephen,
> You can make it from the components of HL-5 which could
> be obtained from Himedia(INDIA)
>
>
>
>
> Regards,
> Nimi Vashi,
> MS Final Year Biochemistry,
> The M S University Of Baroda,
> Vadodara,
> Gujarat,
> Indai
>
> On Wed, Jan 6, 2010 at 2:56 AM, Stephen Burrows < [log in to unmask] >
> wrote:
>
> Has anyone purchased media from Formedium, a company out of the UK? We
> have requested HL-5 and LoFlo medium for testing in-house. But, it appears
> that Formedium does not have an office in the US and that we would have to
> act as importees (at additional cost) to get the media into the US. Has
> anyone gone through this process? And if so is the associated cost and
> issues dealing with the USDA worth the effort? The cost of media,
> depending on shipping charges, appears to be >than 60% from what we are
> presently doing now.
>
> Would be thankful for any insight anyone might have on this subject.
>
> Stephen Burrows
> PhD Candidate
> Clark University
> Department of Biology
> 950 Main Street
> Worcester, MA 01610
>
>
>
> --
> Nimi K Vashi,
> Dr K.G.Naik Department Of Biochemistry,
> The Maharaja Sayajirao University Of Baroda,
> Vadodara,
> Gujarat,
> India.
>
>
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