Hi Rob- We (i.e. Patrick Suess) have used MG132, works at 6 micromolar (and probably lower) on lysates, 12 micromolar to affect whole cells Lactacystin had no effect on whole cells. see Patrick's J. Cell Science 2017 paper for details cheers Richard Richard Gomer Department of Biology Texas A&M University 301 Old Main Drive College Station, TX 77843-3474 979 458 5745 ________________________________________ From: DICTY [[log in to unmask]] on behalf of Rob Kay [[log in to unmask]] Sent: Monday, March 5, 2018 11:38 AM To: [log in to unmask] Subject: [DICTY] Proteasome inhibitors Does anybody have recent experience of proteosome inhibitors with Dicty? Which ones work (and at what concentration) and which do not? This question was last asked in 2003, but perhaps there has been progress since then. Thank you friends and colleagues for any help! Rob Kay Robert Kay, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, England Phone, Office:+ 1223 267039 (01223 267039 from UK) Phone, Lab: + 1223 267386 (01223 267386 from UK) FAX: + 1223 268300 (01223 268300 from UK) Web page: https://urldefense.proofpoint.com/v2/url?u=http-3A__www2.mrc-2Dlmb.cam.ac.uk_groups_rrk_index.html&d=DwIFAg&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=ttDm5-LCkQhmuQw-kW4alr5td7ZeT-2dhvIYFHKaRDA&s=FDNp5leiil7GabSB_ynUA51Unk5Uyqk7Efh1yLoyn4g&e=
