Many thanks to all of you for the helpful comments!

Chris

Christian Hammann

Heisenberg research group ribogenetics

Faculty of Biology

Technical University Darmstadt

Schnittspahnstr. 10

64287 Darmstadt

t +49 6151 – 16 3955

f +49 6151 – 16 7440

e [log in to unmask]


On Jul 21, 2010, at 7:59 PM, Stephen D. Burrows wrote:

> Dear Chris,
>
> We have been using the Formedium HL-5 without glucose and  
> supplementing
> with a molecular biology grade D-glucose.  We get a deep caramel  
> almost
> brown color after autoclaving.  I have not run any doubling time
> experiments but the AX2 cells reach confluence within 4-5 days with  
> this
> media vs. 5-6 days with our older homemade HL-5.  And some of the  
> growth
> curves that I have done also tend to show slightly higher numbers with
> shorter time points.
>
> The media used does seem to have an influence on the growth of AX2  
> cells.
> AX2 cells grow slower in the FM-vitamin supplemented media extended  
> the
> growth curves out at least 24-36 hours vs the HL-5 media in our lab.
>
> Hope this is helpful.
>
> Best regards,
>
> Stephen D. Burrows
> Department of Biology
> Clark University
> Phone: 508-942-1056
>
>> Dear all,
>>
>> since more than two months we observe very irregular growth of our
>> wild type AX2:
>> the doubling time varies as it seems at random between 10 and more
>> than 40 hours.
>>
>> All the usual parameters including temperature, medium (we use
>> Formedium HL5 medium with glucose), water, residual detergent in
>> flasks from the dish washer, pH, light or culture density have been
>> checked and they all do not seem to be the reason for this behavior.
>>
>> My questions are the following:
>>
>> Is this also observed in other labs?
>> What is the usual doubling time of AX2 in your hands?
>> How constant is that doubling time?
>> How many of you have tried to supplement the medium with vitamins, as
>> stated in the technical comments on Dictybase, and how successful was
>> that?
>>
>> Your input is much appreciated,
>>
>> Chris
>>
>>
>> Christian Hammann
>>
>> Heisenberg research group ribogenetics
>>
>> Faculty of Biology
>>
>> Technical University Darmstadt
>>
>> Schnittspahnstr. 10
>>
>> 64287 Darmstadt
>>
>> t +49 6151 – 16 3955
>>
>> f +49 6151 – 16 7440
>>
>> e [log in to unmask]
>>
>>