Hello, I have a question for all. I am relatively new to using Dicty.
I had obtained Dicty from the Dictybase stocks & properly grew them up as directed.
Due to some unforeseen instances, I had to be away from lab for a few days and as a result, my axenic cultures (in HL5 medium) have become confluent. I am growing them to both store permanently and to do a bacterial co-culture from log-phase growth
I know that at some point they start to differentiate at a certain density. Is it possible to cut these cells into a new fresh diluted media for a new culture for me to use or if once they differentiate, they are no longer useful.
Thank you
~john