Dear Chris,

We have been using the Formedium HL-5 without glucose and supplementing
with a molecular biology grade D-glucose.  We get a deep caramel almost
brown color after autoclaving.  I have not run any doubling time
experiments but the AX2 cells reach confluence within 4-5 days with this
media vs. 5-6 days with our older homemade HL-5.  And some of the growth
curves that I have done also tend to show slightly higher numbers with
shorter time points.

The media used does seem to have an influence on the growth of AX2 cells.
AX2 cells grow slower in the FM-vitamin supplemented media extended the
growth curves out at least 24-36 hours vs the HL-5 media in our lab.

Hope this is helpful.

Best regards,

Stephen D. Burrows
Department of Biology
Clark University
Phone: 508-942-1056

> Dear all,
>
> since more than two months we observe very irregular growth of our
> wild type AX2:
> the doubling time varies as it seems at random between 10 and more
> than 40 hours.
>
> All the usual parameters including temperature, medium (we use
> Formedium HL5 medium with glucose), water, residual detergent in
> flasks from the dish washer, pH, light or culture density have been
> checked and they all do not seem to be the reason for this behavior.
>
> My questions are the following:
>
> Is this also observed in other labs?
> What is the usual doubling time of AX2 in your hands?
> How constant is that doubling time?
> How many of you have tried to supplement the medium with vitamins, as
> stated in the technical comments on Dictybase, and how successful was
> that?
>
> Your input is much appreciated,
>
> Chris
>
>
> Christian Hammann
>
> Heisenberg research group ribogenetics
>
> Faculty of Biology
>
> Technical University Darmstadt
>
> Schnittspahnstr. 10
>
> 64287 Darmstadt
>
> t +49 6151 – 16 3955
>
> f +49 6151 – 16 7440
>
> e [log in to unmask]
>
>