dictyNews

Electronic Edition

Volume 48, number 13

July 22, 2022



Please submit abstracts of your papers as soon as they have been

accepted for publication by sending them to [log in to unmask]

or by using the form at

http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit.



Back issues of dictyNews, the Dicty Reference database and other

useful information is available at dictyBase - http://dictybase.org.



Follow dictyBase on twitter:

http://twitter.com/dictybase





=========

Abstracts

=========







Calcium responses to external mechanical stimuli in the multicellular 

stage of Dictyostelium discoideum



Hidenori Hashimura, Yusuke V. Morimoto, Yusei Hirayama, 

Masahiro Ueda



Scientific Reports 12: 12428 (2022)

https://www.nature.com/articles/s41598-022-16774-3



Calcium acts as a second messenger to regulate many cellular 

functions, including cell motility. In Dictyostelium discoideum, the 

cytosolic calcium level oscillates synchronously, and calcium waves 

propagate through the cell population during the early stages of 

development, including aggregation. In the unicellular phase, the 

calcium response through Piezo channels also functions in 

mechanosensing. However, calcium dynamics during multicellular 

morphogenesis are still unclear. Here, live imaging of cytosolic 

calcium revealed that calcium wave propagation, depending on cAMP 

relay, disappeared at the onset of multicellular body (slug) formation. 

Later, other forms of occasional calcium bursts and their propagation 

were observed in both anterior and posterior regions of migrating 

slugs. This calcium signaling also occurred in response to mechanical 

stimuli. Two pathways—calcium release from the endoplasmic 

reticulum via IP3 receptor and calcium influx from outside the cell—

were involved in calcium signals induced by mechanical stimuli. These 

data suggest that calcium signaling is involved in mechanosensing in 

both the unicellular and multicellular phases of Dictyostelium 

development using different molecular mechanisms.





Submitted by Yusuke Morimoto [[log in to unmask]]

=======================================================

[End dictyNews, volume 48, number 13]