Yes, Harry MacWilliams did this

Dev Genes Evol. 1999 Jan;209(1):63-8.
Green fluorescent proteins with short half-lives as reporters in Dictyostelium discoideum.
Deichsel H1, Friedel S, Detterbeck A, Coyne C, Hamker U, MacWilliams HK.

We describe two modifications of the popular reporter green fluorescent protein (GFP) which have short half-lives in our system, the cellular slime mould Dictyostelium discoideum. One of these bears an N-terminal ubiquitin; this GFP was originally planned to be a substrate of the "N-end-rule" pathway, but deubiquitination does not seem to occur, and a degradation by the UFD (ubiquitin-fusion-degradation pathway seems more probable. The protein half-life is about 3-5 h. The second construct has an N-terminus derived from the L11 ribosomal protein; it is transported to the nucleus and broken down much more rapidly than the ubiquitin fusion (protein half-life about 30 min). We show examples of the use of these reporters in the study of gene expression in Dictyostelium.
PMID: 9914420 

cheers

Richard Gomer


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From: DICTY [[log in to unmask]] on behalf of Nicole Gruenheit [[log in to unmask]]
Sent: Tuesday, November 18, 2014 8:12 AM
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Subject: [DICTY] destruction/degeneration sequence motifs/signals in Dicty

Does anybody have experience with destruction/degeneration sequence motifs/signals in Dicty?  I want to make "labile" promoter::reporter gene (e.g. GFP/RFP) constructs where the GFP/RFP protein is rapidly degraded (e.g. by ubiquitin/proteosome pathway).  Thank you for your help, Nicole and Thomas