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Date: | Tue, 17 Dec 2013 14:59:17 -0600 |
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Hello, Jon,
I am glad you are using Dicty as a teaching tool in Pomona.
For your purposes, staining with crystal violet or Coomasie may be the most appropriate (with fixation, I think).
http://dictybase.org/ListServ_archive/listserv_archive_staining.html
See the section from Dale Hereld.
-Ricky
On Dec 17, 2013, at 11:45 AM, Jon Moore <[log in to unmask]> wrote:
> Last spring we started running a dicty motility teaching lab for an intro cell
> biology/ cell chemistry class. We've been using a modification of the under-agar
> assay, and are happy with the lab with one major exception: it is very difficult
> for our students to see and and identify the cells with the limited microscopic
> power we have. We're using dissecting microscopes with 50x total power, of which
> we have 19 research-grade. We have considered investing in better microscopes,
> but there is little likelihood that we'll get 9 or more of these without outside funding.
> We've tweaked the lighting about as much as possible. Does anyone have any
> suggestions as to how we might better visualize them, including ideas like good
> stains? We're running this as an end-point assay, so if the stain kills them once
> they've migrated, that's Ok.
>
> Thanks and peace,
>
> Jon Moore
> assistant professor, Pomona College
> 909-621-8607
> [log in to unmask]
>
> (Yes, I've met almost none of you. No one at Pomona works on dicty;
> this is an outgrowth of a lab that started with a breast cancer cell line.)
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