Hi all,



we have a codon-optimized APEX2 coding sequence construct, but have not had time to make use of it yet. I’d be happy to provide it to those who would like to try it out.



- Peter



Peter Thomason

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> On 9 Jul 2019, at 10:33, Markus Maniak <[log in to unmask]> wrote:

> 

> Funny enough, we were just making plans for constructing an APEX2 that is targeted to the luminal side of the ER membrane. By having a signal sequence at the N-terminus, we hope that it is codon-optimized enough to be translated properly.

> 

> Kind regards, Markus Maniak

> 

> 

> -----Ursprüngliche Nachricht-----

> Von: DICTY <[log in to unmask]> Im Auftrag von Ryan Gawryluk

> Gesendet: Freitag, 5. Juli 2019 17:29

> An: [log in to unmask]

> Betreff: Re: [DICTY] Inquiry APEX2 Dicty

> 

> Hi Lyudmil,

> 

> I’m also hoping to use APEX. I don’t think anyone has reported using it, though I expect codon usage will be important, as it was for BioID.

> 

> Ryan

> 

>> On Jul 5, 2019, at 08:11, Lyudmil Raykov <[log in to unmask]> wrote:

>> 

>> Dear all,

>> 

>> I’d like to use the engineered peroxidase APEX2 in D. discoideum in order to seek for potential proximal partners of my protein of interest.

>> 

>> Therefore, I have two questions:

>> 

>> 1. Has anyone used APEX2 in D. discoideum cells?

>> 

>> 2. Does anyone know, whether codon bias correction is necessary for the usage of APEX2 in D. discoideum?

>> 

>> Thanking you for your time.

>> 

>> Kind regards,

>> 

>> Lyudmil Raykov