Hi Stefan,

We are quite happy with our Bio-Rad electroporator. Using 1 mm cuvettes at
0.85 kV/25 uF (2 pulses with 5 seconds break between pulses) we get nice
results.

Hope this helps,

Sascha

-- 
Sascha Thewes, PhD
Institute for Biology - Microbiology
Department of Biology, Chemistry, Pharmacy
Freie Universität Berlin
Königin-Luise-Str. 12-16
14195 Berlin
Germany

Phone: +49-(0)30-838-53373
Fax: +49-(0)30-838-57773
Email: [log in to unmask]


Am 10.02.2009 20:55 Uhr schrieb "Stefan Pukatzki" unter
<[log in to unmask]>:

> 
> Hi Everyone,
> 
> We are in the market for a new electroporator, and are wondering if
> anyone out there is extremely happy with their instrument to transform
> amoebae. The choices are limited (Bio-Rad Gene Pulser Xcell Total
> system, The Eppendorf Multiporator, BTX's ECM 830, or Thermo
> Scientific's Celljec T Pro), but if anyone has any recommendations or
> reservations, we would like to hear about it.
> 
> Thank you,
> Stefan
> 
> Stefan Pukatzki, Ph.D.
> Assistant Professor of Medical Microbiology & Immunology
> University of Alberta
> 1-63 Medical Sciences Building
> Edmonton, Alberta T6G 2H7
> 
> 
> 
> 
> phone:  (780) 492-0904
> fax:        (315) 875-2031
> 
> http://www.ualberta.ca/~pukatzki/labpage/Welcome.html
>