DICTY Archives

December 2009, Week 2

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Subject:
Re: I need help
From:
Ruth Furukawa <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Wed, 9 Dec 2009 17:31:23 -0500
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Dear Herb,

Do you want this little bit of DNA to be expressed as a fusion protein to
GFP or be expressed as a peptide?

Ruth

> Hello friends,
>
> I want to put a small DNA sequence (40 bp) in front of and behind a
> GFP cassette.  I want the sequence before the GFP to be transcribed
> and GFP expressed.  Likewise I'd like the DNA sequence after GFP to
> also be transcribed.  Can anyone help me?  What vector (that can
> transform dicty) should I use?
>
> Thanks.
>
> Herb Ennis
>
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-- 
Ruth Furukawa
Assistant Research Scientist
Dept. Cellular Biology
706.542.3338 (Telephone)
706.542.4271 (Fax)

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