Dear Sandrine,

Other than the published methods, here is one 'non-invasive' possibility.
If you have a cell sorter, you could take a growing population, sort cells
by size and pick those at the extreme left or extreme right of the
distribution for initiating the next round of growth.

Good luck,

Vidya


> Dear Dicty community,
>
> I am trying to synchronize dicty with a non invasive method. I check cell
> density by flow cytometry and I am now trying BrdU staining for
> confirmation. I have thus 2 questions:
>
> - do you have some tips for synchronization ? I observe a 3h lag before
> exponential growth for both cells taken just after mitosis and cells well
> stuck to the bottom (expected to be in G2 phase), which is not coherent
> with what expected...
>
> - does anyone use Dako's antibodies ? I tried the protocol from Muramoto
> and Chubb, 2011, complemented by indications (like buffers and times) from
> Dolbeare, 1994. However, it does not work for me..
>
>
> Thanks in advance for your help !
>
>
>
> PS: if the non-invasive synchronisation protocole works, I will be pleased
> to share it with the community !
>
> --
> This message has been scanned for viruses and
> dangerous content by MailScanner, and is
> believed to be clean.
>