Dear Jonathan,

I did some experiments a long time ago treating cells with 0.2 and 
0.4 M sorbitol. They could deal with 0.2 M, i.e. they freeze, shrink 
and round up, but after about 1h they regain shape and motility. With 
0.4 M sorbitol they do not recover and lyse after 1 to 2 hours.

Best wishes,
Ludwig


At 12:46 18.10.2021, you wrote:
>Dear All,
>Does anyone have an idea what the limits for the osmotic shock a 
>Dicty cell can deal with and still function in some capacity?  Is 
>1.2M out of the question?
>Thanks,
>Jonathan
>---
>
>Professor Jonathan Chubb
>
>Wellcome Trust Senior Fellow
>
>MRC Laboratory for Molecular Cell Biology
>
>University College London,
>
>Gower Street,
>
>London
>
>WC1E 6BT