I ran into this problem earlier this year.  I consulted composition  
tables published by BD for all of their peptones, and found that  
Proteose Peptone No. 3 is most similar to Thiotone E peptone.  Thus, I  
now make HL5 with a 50:50 mixture of Proteose Peptone No. 2 (#212120)  
and Proteose Peptone No. 3 (#211693).  Cells seem pretty happy in this  
medium.  I have also had good luck with the pre-made stuff from  
Formedium, but it's difficult to get because of customs issues.

Good luck!

Dan Dickinson
Stanford University


On Dec 10, 2010, at 8:54 AM, Petra Fey wrote:

> Dear Maria,
>
> This is a question that's best suited for the ListServ, as it should  
> be of interest to the whole Dicty community. To write to the  Dicty  
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> Good luck in your endeavor, but I'm sure there are people out there  
> who are facing (or faced) the same problem, and sooner or later it  
> concerns many.
>
> Best wishes,
> Petra
>
> On Dec 10, 2010, at 10:22 AM, Maria Abad wrote:
>
>> Hello,
>>
>> Thiotone E peptone (BBL # 212302) has been discontinued. Could you  
>> recommend  a substitute product to make HL5  medium?