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November 2019, Week 1

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Subject:
NBRP-slime molds: CRISPR/Cas9 nickase vectors
From:
Yoichirou Kamimura <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Wed, 6 Nov 2019 17:33:39 +0900
Content-Type:
text/plain
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text/plain (28 lines) 
Dear all,

 

We are happy to inform you of the new CRISPR/Cas9 nickase vectors which were deposited by Dr Tetsuya Muramoto at Toho University. 

https://urldefense.proofpoint.com/v2/url?u=https-3A__journals.plos.org_plosone_article-3Fid-3D10.1371_journal.pone.0224128&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=7JL-Bf-W-3Qm3bh7Q6y43Ud58OZhu6VvAbvOEtuKSwY&s=lQ6nhfcH32OcAINJG70JWlyx7FkinaPFIjj3nq7xBLs&e=  



G90484, pTM1331: https://urldefense.proofpoint.com/v2/url?u=https-3A__nenkin.nbrp.jp_clone_plasmid_detail_G90484&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=7JL-Bf-W-3Qm3bh7Q6y43Ud58OZhu6VvAbvOEtuKSwY&s=fOJ_lzBVcg_PJKKLczPkTFoITmMRHrCVjzueyrBYaqM&e= 

G90485, pTM1355: https://urldefense.proofpoint.com/v2/url?u=https-3A__nenkin.nbrp.jp_clone_plasmid_detail_G90485&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=7JL-Bf-W-3Qm3bh7Q6y43Ud58OZhu6VvAbvOEtuKSwY&s=FawYLvvnwtcftCi8XxuMdE3GEeJ1SJZ-3W-emsDD5W0&e= 

G90486, pTM1372: https://urldefense.proofpoint.com/v2/url?u=https-3A__nenkin.nbrp.jp_clone_plasmid_detail_G90486&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=7JL-Bf-W-3Qm3bh7Q6y43Ud58OZhu6VvAbvOEtuKSwY&s=MvnE90qBBWfG0cr0PFfImrWKXRoD5B0-svd8i497jqM&e= 

G90487, pTM1544: https://urldefense.proofpoint.com/v2/url?u=https-3A__nenkin.nbrp.jp_clone_plasmid_detail_G90487&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=7JL-Bf-W-3Qm3bh7Q6y43Ud58OZhu6VvAbvOEtuKSwY&s=5KbGNRaXEwxJPDZ23MxnWpJNmSLa5tGNn4intJ_3I7M&e= 



These vectors were used to make deletions and introduce precise point mutations,and are available from NBRP-slime molds Japan. https://urldefense.proofpoint.com/v2/url?u=https-3A__nenkin.nbrp.jp_locale_change-3Flang-3Den&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=7JL-Bf-W-3Qm3bh7Q6y43Ud58OZhu6VvAbvOEtuKSwY&s=96aXHz4Jhv4Fudw97jw8dGfEvJKGkckfMIaMUOI7NCw&e=  



We hope that these new tools will help your work. 



Best wishes, 



National BioResource Project Japan (NBRP)-slime molds

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Yoichiro KAMIMURA



RIKEN　Center for Biosystems Dynamics Research (BDR)

Cell Signaling Dynamics Team (Senior researcher)

6-2-3, Furuedai, Suita city, Osaka, 

Japan, 565-0874

Tel: +81-70-6800-3894

[log in to unmask]

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