Hello, Mona,



The first sequenced Dicty genome is from an isolate of AX4. There’s some debate as to the ancestry of the different strains that have evolved in different labs - arguably, AX4 descended from AX3, but even with the same designation, an AX3 from one part of the world is not necessarily genetically identical to that from another. But for the most part, what is in NCBI and Dictybase is assembed from a carefully curated clonal isolate AX4. 



Now, what is rlnA? A keyword search in NCBI for that gene name doesn’t turn anything up. Could you mean rnlA? Which is encoded on the mitochondrion. 



Note also that rRNA genes are encoded on extrachromosomal palindromic elements in Dicty. Nucleic Acids Res. 2003 May 1;31(9):2361-8.







> On Feb 9, 2017, at 3:51 AM, mona saad <[log in to unmask]> wrote:

> 

> Bonjour,

> I'm wondering if AX3 strain genome of dicty is registered in NCBI, because when trying to blast rRNA sequence of rlna gene with RNArefseq, I don't find the gene sequence, so maybe because AX3 is not registered in NCBI? any idea?

> thank you