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| Date: | Tue, 27 Mar 2018 19:15:57 +0000 |
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An important consideration is that many of these dyes will be pumped out by the transporters, and the signal weakens as the cells sit. I seem to recall someone doing a GFP-histone fusion that can be used in this manner.
> On Mar 25, 2018, at 11:31 PM, 久保原禅 <[log in to unmask]> wrote:
>
> Dear Dr.
> You can stain the nucleus in living dicty cells with hoechst33342 (for example, final conc. 1 microg/ml for 30 min).
> But the dye stains the cell walls of stalk cells (and probably spores), too. be careful.
>
> Best wishes,
>
> Yuzuru Kubohara, Ph.D.
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> 2018-03-25 2:31 GMT+09:00 mona saad <[log in to unmask]>:
> Dear all,
> could you recommend for me a fluorescent dye that color the nucleus in dicty in order to do imaging in living cells?
> Thank you
>
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