Hi Muatasem,
I have applied similar approach to clone a novel gene (vwkA) from
Dictyostelium during development [MBC (2005) 16:2248-2262].
I used TRIzol to isolate RNA from ten million cells.
Good luck!
Venk
-----------------------------------
Venkaiah Betapudi, Ph.D.
Assistant Professor of Molecular Medicine
Department of Cell Biology (NC10)
Cleveland Clinic Foundation
9500 Euclid Avenue
Cleveland, OH 44195
Tel 216-445-2359
Fax 216-444-9404
E-mail [log in to unmask]
           [log in to unmask]

-----Original Message-----
From: DICTY [mailto:[log in to unmask]] On Behalf Of
Ubeidat, Muatasem
Sent: Thursday, August 09, 2012 10:55 AM
To: [log in to unmask]
Subject: [DICTY] RNA extraction

Hi,

We are interested in RNA isolation from different developmental stages
of Dictyostelium in particular slugs and later stages for the purpose of
cDNA synthesis. Any suggestion of methods and/or Kits will be greatly
appreciated.

Thanks

-Muatasem

MUATASEM UBEIDAT, Ph.D.
Associate Professor of Genetics and Molecular Biology
Southwestern Oklahoma State University
Department of Biological Sciences
Molecular Biology and Genetics Lab
SCI 213
100 Campus Drive
Weatherford, OK 73096
Tel 1-580-774-3298
Fax 1-580-774-7140
E-mail: [log in to unmask]

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