dictyNews
Electronic Edition
Volume 46, number 9
March 20, 2020
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Abstracts
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Transcriptional responses of Dictyostelium discoideum exposed to
different classes of bacteria
Otmane Lamrabet1*, Astrid Melotti1, Frédéric Burdet2, Nabil Hanna3,
Jackie Perrin1, Jahn Nitschke3, Marco Pagni2, Hubert Hilbi4,
Thierry Soldati3, Pierre Cosson1
1Faculty of Medicine, Department of Cell Physiology and Metabolism,
University of Geneva, Geneva, Switzerland
2SIB, Swiss Institute of Bioinformatics, Vital-IT group, Amphipôle building,
Lausanne, Switzerland
3Faculty of Science, Department of Biochemistry, University of Geneva,
Switzerland
4Faculty of Medicine, Institute of Medical Microbiology, University of
Zurich, Switzerland
*Author for correspondence ([log in to unmask])
Frontiers in Microbiology, Infectious Diseases, 2020 11:410.
doi: 10.3389/fmicb.2020.00410
Dictyostelium discoideum amoebae feed by ingesting bacteria, then killing
them in phagosomes. Ingestion and killing of different bacteria have been
shown to rely on largely different molecular mechanisms. One would thus
expect that D. discoideum adapts its ingestion and killing machinery when
encountering different bacteria. In this study, we investigated by RNA
sequencing if and how D. discoideum amoebae respond to the presence
of different bacteria by modifying their gene expression patterns. Each
bacterial species analyzed induced a specific modification of the
transcriptome. Bacteria such as Bacillus subtilis, Klebsiella pneumoniae,
or Mycobacterium marinum induced a specific and different transcriptional
response, while Micrococcus luteus did not trigger a significant gene
regulation. Although folate has been proposed to be one of the key
molecules secreted by bacteria and recognized by hunting amoebae, it
elicited a very specific and restricted transcriptional signature, distinct from
that triggered by any bacteria analyzed here. Our results indicate that
D. discoideum amoebae respond in a highly specific, almost non-overlapping
manner to different species of bacteria. We additionally identify specific sets
of genes that can be used as reporters of the response of D. discoideum to
different bacteria.
submitted by: Otmane Lamrabet [[log in to unmask]]
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