DICTY Archives

August 2012, Week 2

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Subject:
Re: RNA extraction
From:
Dan Dickinson <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Thu, 9 Aug 2012 12:03:14 -0400
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Hi Muatasem, 

I isolated total RNA using Trizol, following the manufacturer's protocol.  It works beautifully. 

Best, 
Dan Dickinson

On Aug 9, 2012, at 10:55 AM, "Ubeidat, Muatasem" <[log in to unmask]> wrote:

> Hi,
> 
> We are interested in RNA isolation from different developmental stages of Dictyostelium in particular slugs and later stages for the purpose of cDNA synthesis. Any suggestion of methods and/or Kits will be greatly appreciated.
> 
> Thanks
> 
> -Muatasem
> 
> MUATASEM UBEIDAT, Ph.D.
> Associate Professor of Genetics and Molecular Biology
> Southwestern Oklahoma State University
> Department of Biological Sciences
> Molecular Biology and Genetics Lab
> SCI 213
> 100 Campus Drive
> Weatherford, OK 73096
> Tel 1-580-774-3298
> Fax 1-580-774-7140
> E-mail: [log in to unmask]

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