We tried quite a few a number of years ago examining their effect on a  
number of proteins that we demonstrated genetically and showed by  
Westerns were being targeting for degradation by the proteosome.   
Nothing we tried in vivo worked, including MG132.  Don't recall the  
last- it's been over a decade.  By bet it's an efflux problem because  
of the industrial strength concentrations we tried, but it could be  
permeability.

Best-

Rick

On Nov 6, 2009, at 6:11 AM, Thomas Egelhoff wrote:

> Hi Dicty folks:
> We have some studies where we would like to block proteolysis in  
> live Dicty cells.  Has anyone tried MG132 or other proteosome  
> inhibitors on Dicty?  I see no pubmed refs to MG132, I am wondering  
> does it not work or has no one tried it?
> Thanks,
> Tom Egelhoff

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