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January 2013, Week 5

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"John B. Biggins" <[log in to unmask]>
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Date:
Thu, 31 Jan 2013 12:34:42 -0500
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It should be noted that I am using these plates solely for growing AX2 strains for a bacterial predation protocol.  No cloning, development,  etc.

On Jan 31, 2013, at 11:41 AM, Gerda Saxer wrote:

> Before using Formedium we consistently used the same peptone (e.g. #3 or #2) for an experiment.  Changing the peptone seemed to effect the wild clones in particular.  
> 
> Gerda
> 
> 
> On Jan 31, 2013, at 10:27 AM, Joan Strassmann wrote:
> 
>> Generally switching media can be risky, particularly in the middle of an experiment. We have increasingly turned to the very uniform product from Formedium.
>> Joan
>> On Jan 31, 2013, at 8:04 AM, John B. Biggins wrote:
>> 
>>> Dicty newbie here:  
>>> 
>>> Anyone see any difference in using bacto peptone instead of proteose peptone in SM plates?
>>> 
>>> Thanks
>> 
>> _________________________________
>> Joan E. Strassmann
>> Professor of Biology
>> 
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