Dear Cynthia,
Unless there is a reason not to use detergents, classical lysis  
buffers work fine. You could for example lyse in 0.5% TX100.
Best
Pierre
On 11 avr. 11, at 20:06, Damer, Cynthia Kay wrote:

> Dear Colleagues,
>
> Does someone have a protocol for purifying GST-fusion proteins from
> Dictyostelium cells?  I'm most concerned with finding a way to lyse  
> the
> cells.  I used to use a French Press, but no longer have access to  
> one.
>
> Thanks,
>
> Cynthia
>
> Cynthia K. Damer
> Associate Professor
> Biology Department
> Brooks Hall Room 229
> Central Michigan University
> Mount Pleasant, MI 48859
> (989) 774-3455

Pierre Cosson
Centre Medical Universitaire
Dpt of Cell Physiology and Metabolism
1 rue Michel Servet
CH1211 Geneva 4, Switzerland
Email:[log in to unmask]
Tel. (41) 22 379 5293
Fax (41) 22 379 5338