Hi David,

I've done quite a few of these assays (see also references below). I start them by adding a couple of sori to one end of the streak.  The speed seems to be a function of both their growth rate and motility.

Cheers,
John

Horn, E. G. 1971. Food competition among cellular slime molds (Acrasieae). Ecology 52:475-484.

Kuserk, F. T. 1980. The relationship between cellular slime-molds and bacteria in forest soil. Ecology 61:1474-1485.

Eisenberg, R. M., L. E. Hurd, and R. B. Ketcham. 1989. The cellular slime-mold guild and its bacterial prey - growth-rate variation at the interspecific and intraspecific levels. Oecologia 79:458-462.

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John D. Reeve
Associate Professor
Department of Zoology
1125 Lincoln Drive
Southern Illinois University
Carbondale, IL 62901-6501
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Free statistics textbook with SAS and R examples: 
https://urldefense.proofpoint.com/v2/url?u=https-3A__biostatisticsjdreeve.com&d=DwIFAg&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=ODKNUk5ui07LfKT-br0Tri-Y00OBhJ9rZrOrf7Z-Vvk&s=3cpIvgVXIKizABRi5WZKvEq6fAE2xyPers5W66-wHqI&e= 

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-----Original Message-----
From: DICTY [mailto:[log in to unmask]] On Behalf Of Knecht, David
Sent: Tuesday, March 14, 2017 2:40 PM
To: [log in to unmask]
Subject: [DICTY] Bacterial streak Dicty growth assay

I have a vague recollection of a paper where someone used a streak assay to compare rates of consumption of different types of bacteria by Dictyosteleids.  My mental image is an agar plate with several linear streaks of bacteria.  The Dicty were inoculated at one end and the rate at which they moved up and cleared the line used as a measure of Dicty growth.  Perhaps it was a way to compare whether they consumed that type of bacteria at all.   Can anyone point me toward an assay like that?  It sounds simple, but I suspect there are tricks.  Thanks- Dave

Dr. David Knecht
Professor , Department of Molecular and Cell Biology 
University of Connecticut	
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200