We had success in Lesly Temesvari's lab with detecting a single, N-terminal(sorry, not C-terminal) HA tag with Zymed, Rabbit anti-HA (Invitrogen Cat.# 71-5500). It works well with whole cell lysates on Western blots. We tried several, and this is the only one that worked well for us. We used it at 1:125. 

Terri F. Bruce, Ph.D.
Research Assistant Professor
Manager, Jordan Hall Imaging Facility
Department of Biological Sciences
Clemson University
132 Long Hall
Clemson, SC 29634
Phone: (864) 656-1264, FAX: (864) 656-0435
E-mail: [log in to unmask]


-----Original Message-----
From: DICTY [mailto:[log in to unmask]] On Behalf Of Ted Cox
Sent: Thursday, August 19, 2010 12:29 PM
To: [log in to unmask]
Subject: [DICTY - 390] HA tags

Dear All,

We've had trouble detecting a single C-terminal HA tag in transformed  
cells, both in cell extracts, and to a lesser extent, with partially  
purified proteins.  We think it may be the primary antibody--there are  
many sources.  Any observations and help would be greatly appreciated.

Ted

Edward Cox
Professor of Molecular Biology
Edwin Grant Conklin Professor of Biology
333 Moffett Lab
Phone: 609-258-3856
Lab Phone: 609-258-3571
Fax: 609 258 6730

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