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November 2016, Week 2

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Subject:
Re: Difficulty in dictyostelium genome sequencing
From:
Richard Gomer <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Sun, 13 Nov 2016 19:30:13 +0000
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Hi-
Back in the Firtel Lab we found that doing the sequencing reaction at a higher temperature helped

Richard Gomer

________________________________________
From: DICTY [[log in to unmask]] on behalf of mona saad [[log in to unmask]]
Sent: Sunday, November 13, 2016 3:30 AM
To: [log in to unmask]
Subject: [DICTY] Difficulty in dictyostelium genome sequencing

Hello everybody,
I'm working in my project on Dictyostelium discoideum, in fact, we are facing a problem due to the richness of dicty genome with AT bases:  we aimed to do sequencing of some sequences we amplified by PCR. We sent our PCR products to GATC Biotech, and we failed to obtain a good sequencing for the most, probably due to the richness of dicty genome in AT bases.. I wonder if anybody has an experience in dicty sequencing? Is there a specific way to do sequencing for AT rich genome (complicated genome)?any advice or idea?
Thank you

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