I believe Annette Müller-Taubenberger had something similar - maybe she 
put it into the Dicty collection?

wolfgang


Am 29.03.2018 um 16:34 schrieb Meili, Rudolf:
> We did that a couple of years ago in Rick Firtel’s lab. We did a mCherry-H2B fusion. I believe it was the mouse gene. Rick retired and I moved on so that plasmid will need to be recreated but it worked well.
>
> Ruedi
>
>> On Mar 27, 2018, at 12:32, Sucgang, Richard S <[log in to unmask]> wrote:
>>
>> An important consideration is that many of these dyes will be pumped out by the transporters, and the signal weakens as the cells sit. I seem to recall someone doing a GFP-histone fusion that can be used in this manner.
>>
>>
>>> On Mar 25, 2018, at 11:31 PM, 久保原禅 <[log in to unmask]> wrote:
>>>
>>> Dear Dr.
>>> You can stain the nucleus in living dicty cells with hoechst33342 (for example, final conc. 1 microg/ml for 30 min).
>>> But the dye stains the cell walls of stalk cells (and probably spores), too. be careful.
>>>
>>> Best wishes,
>>>
>>> Yuzuru Kubohara, Ph.D.
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>>>
>>> 2018-03-25 2:31 GMT+09:00 mona saad <[log in to unmask]>:
>>> Dear all,
>>> could you recommend for me a fluorescent dye that color the nucleus in dicty in order to do imaging in living cells?
>>> Thank you
>>>