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December 2009, Week 1

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Subject:
about cell death after FACS sorting
From:
"Liao, Xin-hua (NIH/NIDDK) [F]" <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Mon, 7 Dec 2009 11:18:50 -0500
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text/plain
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text/plain (6 lines) 
Hi, Everyone,
I am doing a screening with fluorescence-activated cell sorting technique. Even after optimization (plus sucrose in the collection tube suggested by Adam Kuspa, rescue and grow cells on KA plate), only ~10% of cells survive through this physical sorting. This makes large-scale screening more difficult.
The cells were sporulated for 24 hours, and then applied to FACS machine.
Any suggestions for further cell viability are welcome.
Thanks, Xin-Hua Liao

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