Dear Pat,

A priori the simplest test would be to plate your cells on a lawn of  
Klebsiella bacteria (do you have the strains/conditions?), and look  
for colonies with a smooth phenotype (no aggregation). Simple to do,  
quite visual.

Best

Pierre


On 15 oct. 09, at 19:10, Patrick Thorpe wrote:

> Dear All,
>
>  We teach a semester long project based CMB lab for seniors.  One of  
> the three projects we do involves Dicty cAMP receptor mutants.  This  
> is the least experimental of the three experiments we do as we have  
> the students characterize already known mutants.
>
>  We're considering how to modify this experiment to where we could  
> supply a mutagenized culture of Dicty to the students (prob. a  
> nitroguanidine mutation?) and have them isolate a aggregation  
> mutant.  They could then do cAMP response assays and DNA screening  
> of the car and other genes.
>
>  So, I'm a bacterial, yeast, and drosophila guy - any ideas of how  
> to do an easy screen for students?
>
>  Thanks a lot,
>
>  Pat

Pierre Cosson
Centre Medical Universitaire
Dpt of Cell Physiology and Metabolism
1 rue Michel Servet
CH1211 Geneva 4, Switzerland
Email:[log in to unmask]
Tel. (41) 22 379 5293
Fax (41) 22 379 5338