Hi Maria,

By mistake I sent the response to you already to the ListServ, and it got already a couple of responses, pasted below. If you have general Dicty questions you should subscribe, as often important topics are being discussed.

Best wishes,
Petra

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I ran into this problem earlier this year.  I consulted composition tables published by BD for all of their peptones, and found that Proteose Peptone No. 3 is most similar to Thiotone E peptone.  Thus, I now make HL5 with a 50:50 mixture of Proteose Peptone No. 2 (#212120) and Proteose Peptone No. 3 (#211693).  Cells seem pretty happy in this medium.  I have also had good luck with the pre-made stuff from Formedium, but it's difficult to get because of customs issues.

Good luck!

Dan Dickinson
Stanford University
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We have started using proteose peptone 3 in stead of proteose peptone 2 from BD over a year ago when proteose peptone 2 was on a too long backorder, and have not noticed any differences in growth. We now use only proteose peptone 3 in our HL5.

Greetings,

Hanke
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On Dec 10, 2010, at 10:54 AM, Petra Fey wrote:

Dear Maria,

This is a question that's best suited for the ListServ, as it should be of interest to the whole Dicty community. To write to the  Dicty Listserv, send your email to [log in to unmask]

To receive the responses to the ListServ, please subscribe by filling out this form:
http://dictybase.org/db/cgi-bin/dictyBase/colleague/colleagueUpdate

Good luck in your endeavor, but I'm sure there are people out there who are facing (or faced) the same problem, and sooner or later it concerns many.

Best wishes,
Petra

On Dec 10, 2010, at 10:22 AM, Maria Abad wrote:

> Hello,
> 
> Thiotone E peptone (BBL # 212302) has been discontinued. Could you recommend  a substitute product to make HL5  medium?