Hi Kristi and All,

At the Stock Center we now have XL10 Gold and we are just starting to transform the biggest pDM plasmids (that are not Gateway) into these cells. We will do pDM310 next, and if anyone wants us to do a specific plasmid write to [log in to unmask] to push it up the priority list.

Best wishes,
Petra 

On Feb 11, 2013, at 4:24 PM, Kristi Miller wrote:

> Hello!
> 
> I am working with the pDM310 vector. Has anyone designed primers that they know to work well for sequencing? Also, when digesting the constructs that I have made with the pDM310 vector with an insert I sometimes see additional bands that do not make sense... Has anyone else encountered this problem? Is there a chance that the pDM310 vector even after digestion becomes strangely supercoiled?
> 
> Thanks!
> Kristi
> 
> 
> Kristi E. Miller
> Graduate Teaching Assistant 
> Biology Department
> Dow Hall Room 246
> Central Michigan University
> Mount Pleasant, MI 48859
> 
>