Hey Steve,

In this paper:

A defined minimal medium for axenic strains of Dictyostelium
discoideum
J. Franke, R. Kessin
Proc. Nati. Acad. Sci. USA
Vol. 74, No. 5, pp. 2157-2161, May 1977

if you look at the Table 1, they list ~0.3 mM of CaCl2 in HL5.


--
Igor Segota
graduate student
Cornell University

2012/1/18 Steve Charette <[log in to unmask]>:
> Dear Dicty colleagues,
>
> I have a simple question for you...
>
> What is the concentration of calcium in HL5 medium?
>
> Thank you in advance for the answer and the reference if possible.
>
> Steve
> ----------------------------------------------------------------------------
> ----------------------------------------------------------------------------
> Steve Charette, Ph. D.
> Professeur adjoint (Dépt. Biochimie, microbiologie et bio-informatique,
> Université Laval)
>
> [log in to unmask]
>
> WEBSITE: www.amibe.org
>
> PRIMARY AFFILIATION
> Institut de Biologie Intégrative et des Systèmes (IBIS)
> Pavillon Charles-Eugène-Marchand
> 1030, avenue de la Médecine
> Université Laval
> Québec, Qc
> G1V 0A6, CANADA
> Office: 418 656-2131 ext. 6914
> Fax:    418 656-7176
>
> SECONDARY AFFILIATION
> Centre de recherche,
> Inst. Uni. de cardiologie et de pneumologie de Québec
> 2725 Chemin Sainte-Foy
> Québec, Qc
> G1V 4G5, CANADA
> Office: 418 656-8711 ext. 3311