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January 2021, Week 1

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Subject:
Re: Asking about inducible vector
From:
Rob Kay <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Tue, 5 Jan 2021 20:02:42 -0000
Content-Type:
text/plain
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text/plain (22 lines) 
Dear Yiyan,

You should look at Peggy Paschke's paper for faster and more versatile
methods:

Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, et
al. (2018) Rapid and efficient genetic engineering of both wild type and
axenic strains of Dictyostelium discoideum. PLoS ONE 13(5): e0196809.
https://urldefense.com/v3/__https://doi.org/10.1371/journal.pone.0196809__;!!Dq0X2DkFhyF93HkjWTBQKhk!BNqFlwYTLeHtTEYheIBBICuxsZ66c3VtUzeM-wJ-8p71CbrgoGlrZLMWCZnslnDPKl2ITStvqFka6g$ 

Rob

> To whom it may concern,
>
> We are about to make an inducible construct based on pDM359 which has
> worked for us previously.  It has been a while and we are wondering if
> anyone has improved on this system in the interim.  Many thanks!
>
> Best,
> Yiyan
>

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