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July 2019, Week 1

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Subject:
Re: Inquiry APEX2 Dicty
From:
Ryan Gawryluk <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Fri, 5 Jul 2019 15:28:51 +0000
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Hi Lyudmil,



I’m also hoping to use APEX. I don’t think anyone has reported using it, though I expect codon usage will be important, as it was for BioID.



Ryan



> On Jul 5, 2019, at 08:11, Lyudmil Raykov <[log in to unmask]> wrote:

> 

> Dear all,

> 

> I’d like to use the engineered peroxidase APEX2 in D. discoideum in order to seek for potential proximal partners of my protein of interest.

> 

> Therefore, I have two questions:

> 

> 1. Has anyone used APEX2 in D. discoideum cells?

> 

> 2. Does anyone know, whether codon bias correction is necessary for the usage of APEX2 in D. discoideum?

> 

> Thanking you for your time.

> 

> Kind regards,

> 

> Lyudmil Raykov

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