Hi Muatasem,

I have been using a Kit from Amersco (Code N788-Kit) Phenol-Free Total RNA Purification Kit.  It has worked very well and the cDNA obtained downstream has been used for Q-PCR with very good results.

Stephen Burrows
Ph.D. Candidate
Department of Biology
Clark University
Cell: 508-942-1056

________________________________________
From: DICTY [[log in to unmask]] on behalf of Dan Dickinson [[log in to unmask]]
Sent: Thursday, August 09, 2012 12:03 PM
To: [log in to unmask]
Subject: Re: [DICTY] RNA extraction

Hi Muatasem,

I isolated total RNA using Trizol, following the manufacturer's protocol.  It works beautifully.

Best,
Dan Dickinson

On Aug 9, 2012, at 10:55 AM, "Ubeidat, Muatasem" <[log in to unmask]> wrote:

> Hi,
>
> We are interested in RNA isolation from different developmental stages of Dictyostelium in particular slugs and later stages for the purpose of cDNA synthesis. Any suggestion of methods and/or Kits will be greatly appreciated.
>
> Thanks
>
> -Muatasem
>
> MUATASEM UBEIDAT, Ph.D.
> Associate Professor of Genetics and Molecular Biology
> Southwestern Oklahoma State University
> Department of Biological Sciences
> Molecular Biology and Genetics Lab
> SCI 213
> 100 Campus Drive
> Weatherford, OK 73096
> Tel 1-580-774-3298
> Fax 1-580-774-7140
> E-mail: [log in to unmask]