Dear Rob,

We already observed the actin flow by speckle microscopy, but we did not publish yet. Actin shows a retrograde flow in the pseudopod as the cell advances but does not move relative to the substratum.

best regards,

Shige

-------------------------------------------------
Shigehiko YUMURA
Professor of Cell Biology
Graduate School of Sciences and Technology for Innovation, 
Yamaguchi University, 
Yamaguchi 753-8512, Japan
TEL/FAX:+81-83-933-5717
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> 2019/11/20 15:08、Wouter-Jan Rappel <[log in to unmask]>のメール:
> 
> Hi Rob,
> 
> We have recently carried out some speckle microscopy measurements.
> They are not published yet but I would be happy to share and discuss them with you.
> 
> Best,
> 
> Wouter-Jan Rappel
> 
> 
> On 11/19/19 3:28 AM, Rob Kay wrote:
>> Dear All,
>> 
>> Does anybody have experience with speckle microscopy to detect flows of
>> F-actin in Dicty?
>> 
>> I am not aware of any publications on the topic, but with the long history
>> of cytoskeletal research in Dicty, I feel sure somebody must have tried.
>> Would be good to know, even if the experience is negative.
>> 
>> Thanks as always for your help.
>> 
>> Rob
> 
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