dictyNews

Electronic Edition

Volume 49, number 8

March 17 2023



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Abstracts

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Proximity protein labeling In Dictyostelium with engineered ascorbic 

acid peroxidase 2



Jamie A. Takashima2,3, Helena A. Woroniecka2, and 

Pascale G. Charest1,2,*





Journal of Biological Methods 10: e99010002, 2023



To fully understand any cellular process, we not only need to identify 

the proteins implicated, but also how the protein network is structurally 

and spatially organized and changes over time. However, the dynamic 

nature of many protein interactions involved in cellular signaling 

pathways continues to be the bottleneck in mapping and studying 

protein networks. Fortunately, a recently developed proximity labeling 

method using engineered ascorbic acid peroxidase 2 (APEX2) in 

mammalian cells allows the identification of weak and/or transient 

protein interactions with spatial and temporal resolution. Here, we 

describe a protocol for successfully using the APEX2-proximity labeling 

method in Dictyostelium, using the cAMP receptor cAR1 as example. 

Coupled to the identification of the labeled proteins by mass 

spectrometry, this method expands Dictyostelium’s proteomics toolbox 

and should be widely useful for identifying interacting partners involved 

in a variety of biological processes in Dictyostelium.





Submitted by Pascale Charest [[log in to unmask]]

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[End dictyNews, volume 49, number 8]