Hi Wanessa, We are using a kit called RNA magic from bio-budget (www.bio-budget.de). I take 5 million cells for RNA prep. and the yield is about 2µg/µl in a vol. of 15µl suspension. The entire method is also very quick. I used the same kit for isolating RNA from different developmental stages. There may be a silght decrease in RNA yield with late development. Cheers, Ranjani On 1/25/12, Wanessa Cristina DE LIMA <[log in to unmask]> wrote: > Dear all, > > Does anyone have suggestions for good commercially available kits for RNA > extraction? I'm using a NucleoSpin kit from Macherey-Nagel, but yields (at > least in my hands) are normally bad (i use 5-10 million cells/prep). > > Thanks a lot for the help, > Wanessa de Lima > (lab Pierre Cosson, UNIGE, Geneva) > -- Ranjani Dhakshinamoorthy, Doctoral Student, Christian-Albrechts University of Kiel, Germany.