>Hello Muatasem,
    I used RNA-magic(Bio-Budget) to isolate RNA from different development
stages and I followed the protocol available at dictybase for my cDNA
synthesis (deposited by Robin Williams lab)

Best,
Ranjani


 Hi,
>
> We are interested in RNA isolation from different developmental stages of
> Dictyostelium in particular slugs and later stages for the purpose of cDNA
> synthesis. Any suggestion of methods and/or Kits will be greatly
> appreciated.
>
> Thanks
>
> -Muatasem
>
> MUATASEM UBEIDAT, Ph.D.
> Associate Professor of Genetics and Molecular Biology
> Southwestern Oklahoma State University
> Department of Biological Sciences
> Molecular Biology and Genetics Lab
> SCI 213
> 100 Campus Drive
> Weatherford, OK 73096
> Tel 1-580-774-3298
> Fax 1-580-774-7140
> E-mail: [log in to unmask]
>