>Hello Muatasem, I used RNA-magic(Bio-Budget) to isolate RNA from different development stages and I followed the protocol available at dictybase for my cDNA synthesis (deposited by Robin Williams lab) Best, Ranjani Hi, > > We are interested in RNA isolation from different developmental stages of > Dictyostelium in particular slugs and later stages for the purpose of cDNA > synthesis. Any suggestion of methods and/or Kits will be greatly > appreciated. > > Thanks > > -Muatasem > > MUATASEM UBEIDAT, Ph.D. > Associate Professor of Genetics and Molecular Biology > Southwestern Oklahoma State University > Department of Biological Sciences > Molecular Biology and Genetics Lab > SCI 213 > 100 Campus Drive > Weatherford, OK 73096 > Tel 1-580-774-3298 > Fax 1-580-774-7140 > E-mail: [log in to unmask] >