Dear all, The Cell Imaging Facility is glad to announce a special seminar that features Olympus LV200 Bioluminescence Microscope. This instrument is particularly useful for those who work with cells expressing luciferase-tagged proteins. Unlike the IVIS system which acquires images of whole animals, the LV200 is indeed a microscope for cellular work. Related applications include: 1. Do my cells express bioluminescence before I inject them into mice? 2. How do my bioluminescent cells behave in vitro? It would save me time to not have to make a fluorescent version. 3. How bright are my cells, and what is the smallest tumor size I can see if I know the tissue depth and the luminosity of my cells? The system will be under demo here 12/4 - 12/6. I urge you to attend the short seminar and register with Julie Ives (see attached flyer) to test your cells. December 4 (Tue) 2pm Baldwin Auditorium See you there! Regards, Leong -- Teng-Leong Chew, PhD Director, Cell Imaging Facility & Nikon Imaging Center Northwestern University 312-503-2841 On 11/7/12 2:56 PM, "Teng Leong Chew" <[log in to unmask]> wrote: >Hi all, > >I want to bring to your attention a new microscope >that Olympus is about to bring into the Cell Imaging >Facility for testing. > >This microscope (yes, it is a microscope, not a >macroscope like the IVIS or the OV-100) can perform >live cell imaging on cells expressing bioluminescence, >a solution that many of you have been looking for. It >will address issues such as: > >1. Do my cells express bioluminescence before I inject >them into mice? > >2. How do my bioluminescent cells behave in vitro? >It would save me time to not have to make a fluorescent >version. > >3. How bright are my cells, and what is the smallest >tumor size I can see if I know the tissue depth and >the luminosity of my cells? > >Having an in vitro system such as this in the main >imaging facility on the second floor is essential. > >Olympus will leave this system here for a while but >they would like to survey the applications in advance >so that they can prep the demo scope appropriately. > >Please e-mail me (a) if you are interested in testing >this, (b) what would you like to try to do, and (c) >will you have bioluminescence/fluorescence combo, etc. > >I will need your response rather urgently as they are >ready to bring it in right after Thanksgiving. Please >kindly respond. > > >My best, >Leong > >-- >Teng-Leong Chew, PhD > >Director, Cell Imaging Facility & Nikon Imaging Center >Northwestern University >312-503-2841 > >