Dear Agnes,

we have successfully labeled the Dicty nuclear envelope nicely with lamin NE81, Sun1, Nup53 and Nup62.

All the best, Ralph 

Am 31.07.2013 um 10:59 schrieb "Miermont, Agnes" <[log in to unmask]>:

> Dear all,
> I would like to label the nuclear envelop of dicty.
> I have first try using GFP-CAAX but it does not seem to work: I just obtain low cytoplasmic fluorescence.
> I am now trying to label Nucleoporins, I have selected NUP62 as the yeast homolog, Nsp1 tagged with GFP present a nice labeling at the nuclear membrane. I would like to know if anybody has ever try labeling Nucleoporins or have any other idea for nuclear envelop labeling.
> Thank you for your help,
> Agnes
> 
> 
> 
> -------------------------------------------------------------------------------------------------------------------------------
> Agnès Miermont, PhD
> Department of Cell and Developmental Biology
> MRC Laboratory for Molecular Cell Biology
> University College London
> Gower Street
> London
> WC1E 6BT
> ---------------------------------------------------------------------------------------------------------------------------

---------------------------------------------------------

Prof. Dr. Ralph Gräf

Universität Potsdam
Institut für Biochemie und Biologie
ZELLBIOLOGIE

Karl-Liebknecht-Strasse 24-25, Haus 26
14476 Potsdam-Golm
Germany

Tel.: +49-(0)331 9775520
FAX: +49-(0)331 9775522

http://www.bio.uni-potsdam.de/professuren/zellbiologie