Dear all,

The Cell Imaging Facility is glad to announce a special seminar
that features Olympus LV200 Bioluminescence Microscope. This
instrument is particularly useful for those who work with cells
expressing luciferase-tagged proteins. Unlike the IVIS system
which acquires images of whole animals, the LV200 is indeed a
microscope for cellular work. Related applications include:

1. Do my cells express bioluminescence before I inject
them into mice?

2. How do my bioluminescent cells behave in vitro?
It would save me time to not have to make a fluorescent
version.

3. How bright are my cells, and what is the smallest
tumor size I can see if I know the tissue depth and
the luminosity of my cells?

The system will be under demo here 12/4 - 12/6. I urge you to
attend the short seminar and register with Julie Ives (see
attached flyer) to test your cells.

December 4 (Tue)
2pm
Baldwin Auditorium

See you there!


Regards,
Leong

-- 
Teng-Leong Chew, PhD

Director, Cell Imaging Facility & Nikon Imaging Center
Northwestern University
312-503-2841




On 11/7/12 2:56 PM, "Teng Leong Chew" <[log in to unmask]> wrote:

>Hi all,
>
>I want to bring to your attention a new microscope
>that Olympus is about to bring into the Cell Imaging
>Facility for testing.
>
>This microscope (yes, it is a microscope, not a
>macroscope like the IVIS or the OV-100) can perform
>live cell imaging on cells expressing bioluminescence,
>a solution that many of you have been looking for. It
>will address issues such as:
>
>1. Do my cells express bioluminescence before I inject
>them into mice?
>
>2. How do my bioluminescent cells behave in vitro?
>It would save me time to not have to make a fluorescent
>version.
>
>3. How bright are my cells, and what is the smallest
>tumor size I can see if I know the tissue depth and
>the luminosity of my cells?
>
>Having an in vitro system such as this in the main
>imaging facility on the second floor is essential.
>
>Olympus will leave this system here for a while but
>they would like to survey the applications in advance
>so that they can prep the demo scope appropriately.
>
>Please e-mail me (a) if you are interested in testing
>this, (b) what would you like to try to do, and (c)
>will you have bioluminescence/fluorescence combo, etc.
>
>I will need your response rather urgently as they are
>ready to bring it in right after Thanksgiving. Please
>kindly respond.
>
>
>My best,
>Leong
>
>-- 
>Teng-Leong Chew, PhD
>
>Director, Cell Imaging Facility & Nikon Imaging Center
>Northwestern University
>312-503-2841
>
>