Hi Wanessa,
             We are using a kit called RNA magic from bio-budget
(www.bio-budget.de). I take 5 million cells for RNA prep. and the
yield is about 2µg/µl in a vol. of 15µl suspension. The entire method
is also very quick. I used the same kit for isolating RNA from
different developmental stages. There may be a silght decrease in RNA
yield with late development.

Cheers,
Ranjani

On 1/25/12, Wanessa Cristina DE LIMA <[log in to unmask]> wrote:
>  Dear all,
>
> Does anyone have suggestions for good commercially available kits for RNA
> extraction? I'm using a NucleoSpin kit from Macherey-Nagel, but yields (at
> least in my hands) are normally bad (i  use 5-10 million cells/prep).
>
> Thanks a lot for the help,
> Wanessa de Lima
> (lab Pierre Cosson, UNIGE, Geneva)
>


-- 
Ranjani Dhakshinamoorthy,
Doctoral Student,
Christian-Albrechts University of Kiel,
Germany.